LOCUS       OGT-pGEc2               8364 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(7842..7971)
                     /note="3'-ITR"
     misc_feature    complement(7540..7754)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6992..7533
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6171..6193
                     /note="attB2"
     misc_feature    6971..6976
                     /note="BstB1 site"
     misc_feature    6194..6889
                     /note="Super GFP"
     misc_feature    6890..6940
                     /note="AviTag"
     misc_feature    6941..6964
                     /note="His Tag"
     PCR_frame       3011..6170
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..6148
                     /note="OPTIMIZED OGT coding region"
     misc_feature    6149..6170
                     /note="TEV"
     vector          join(6179..8364,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..6178
                     /source="OGT pDONR"
                     /type="Entry Clone"
     source          1..8364
                     /dnas_title="OGT-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCCAGCT CTGTGGGAAA TGTGGCCGAT AGCACCGAGC CCACCAAGAG
     3061 AATGCTGAGC TTTCAGGGAC TGGCCGAGCT GGCTCACAGA GAATATCAGG CCGGCGATTT
     3121 TGAGGCCGCC GAGAGACACT GTATGCAGCT GTGGCGGCAG GAACCCGATA ATACTGGCGT
     3181 GCTGCTGCTC CTGTCCAGCA TCCACTTTCA GTGCCGCAGA CTGGACAGAA GCGCCCACTT
     3241 TAGCACACTG GCCATCAAGC AGAACCCTCT GCTGGCCGAA GCCTACAGCA ACCTGGGCAA
     3301 CGTCTACAAA GAGCGGGGAC AGCTCCAGGA AGCCATCGAG CATTATAGAC ACGCCCTGCG
     3361 GCTGAAGCCC GACTTCATCG ACGGCTATAT CAATCTGGCC GCTGCTCTGG TGGCCGCAGG
     3421 CGATATGGAA GGTGCTGTGC AGGCTTATGT GTCCGCCCTG CAGTACAACC CCGATCTGTA
     3481 TTGTGTGCGG AGCGACCTGG GAAACCTGCT GAAAGCACTG GGAAGGCTGG AAGAGGCCAA
     3541 GGCCTGTTAC CTGAAGGCCA TCGAAACCCA GCCCAACTTC GCCGTGGCTT GGTCTAATCT
     3601 GGGCTGCGTG TTCAATGCTC AGGGCGAGAT CTGGCTGGCT ATCCACCACT TTGAGAAGGC
     3661 CGTGACCCTG GACCCCAATT TCCTGGACGC CTATATTAAC CTGGGGAATG TCCTGAAAGA
     3721 GGCCCGGATC TTCGATAGAG CCGTGGCCGC TTATCTGAGA GCCCTGTCTC TGTCTCCTAA
     3781 TCACGCCGTG GTGCATGGGA ACCTGGCCTG TGTGTATTAC GAGCAGGGCC TGATCGACCT
     3841 GGCCATTGAT ACCTACCGCA GAGCCATTGA GCTGCAGCCT CACTTCCCCG ACGCCTACTG
     3901 TAATCTGGCT AACGCTCTGA AAGAAAAGGG CAGCGTGGCC GAGGCCGAGG ATTGCTATAA
     3961 TACCGCTCTG AGACTGTGCC CCACCCACGC CGATTCTCTG AACAACCTGG CAAACATCAA
     4021 GCGGGAACAG GGCAACATTG AGGAAGCCGT GCGGCTGTAC AGAAAGGCCC TGGAAGTGTT
     4081 CCCTGAGTTT GCCGCCGCTC ACTCTAATCT CGCATCTGTG CTCCAGCAGC AGGGAAAACT
     4141 GCAGGAAGCT CTGATGCATT ACAAAGAAGC CATTCGGATC AGCCCCACCT TTGCCGATGC
     4201 CTACTCCAAT ATGGGCAACA CCCTCAAAGA AATGCAGGAC GTGCAGGGGG CTCTGCAGTG
     4261 CTATACCAGG GCCATCCAGA TCAACCCTGC CTTCGCTGAT GCCCACTCTA ACCTGGCTTC
     4321 CATCCACAAG GACTCCGGCA ACATCCCTGA GGCCATTGCC AGCTACAGAA CCGCCCTGAA
     4381 GCTGAAACCT GATTTCCCAG ATGCCTATTG CAACCTCGCC CACTGCCTGC AGATCGTGTG
     4441 CGACTGGACC GACTACGACG AGCGGATGAA GAAACTGGTG TCCATCGTGG CTGACCAGCT
     4501 GGAAAAGAAC AGACTGCCTA GCGTGCACCC CCACCACTCT ATGCTGTATC CTCTGTCCCA
     4561 CGGCTTTCGG AAGGCTATTG CCGAAAGACA CGGCAACCTG TGCCTGGACA AGATCAACGT
     4621 GCTGCACAAG CCTCCCTACG AGCACCCCAA GGATCTGAAA CTGAGCGACG GCAGACTGAG
     4681 AGTGGGATAC GTGTCCAGCG ACTTCGGCAA CCACCCTACA AGCCACCTGA TGCAGTCTAT
     4741 CCCCGGCATG CACAACCCTG ACAAGTTCGA GGTGTTCTGT TACGCCCTGT CCCCCGACGA
     4801 CGGCACCAAC TTCAGAGTGA AAGTGATGGC TGAGGCTAAC CACTTTATTG ACCTGTCCCA
     4861 GATCCCCTGC AACGGCAAAG CCGCCGATAG AATCCACCAG GACGGAATCC ACATCCTGGT
     4921 CAACATGAAC GGCTACACCA AGGGCGCCAG AAACGAGCTG TTTGCTCTGA GGCCTGCCCC
     4981 TATTCAGGCC ATGTGGCTGG GATATCCTGG AACCAGCGGC GCTCTGTTCA TGGACTACAT
     5041 CATCACCGAC CAGGAAACCT CTCCAGCCGA GGTGGCCGAA CAGTATAGCG AGAAGCTGGC
     5101 CTACATGCCC CACACCTTTT TCATCGGCGA CCACGCCAAC ATGTTCCCCC ACCTGAAGAA
     5161 AAAGGCCGTC ATCGACTTCA AGAGCAACGG GCACATCTAC GACAACCGGA TCGTGCTGAA
     5221 CGGAATCGAT CTGAAGGCTT TTCTGGACTC CCTGCCCGAT GTGAAGATCG TGAAGATGAA
     5281 GTGCCCCGAC GGCGGAGATA ACGCCGACTC TTCTAACACA GCCCTGAACA TGCCCGTGAT
     5341 CCCTATGAAC ACAATCGCCG AGGCTGTGAT CGAGATGATC AACCGGGGGC AGATTCAGAT
     5401 CACCATCAAC GGCTTCTCCA TCTCCAATGG GCTGGCCACC ACACAGATTA ACAACAAGGC
     5461 CGCCACTGGC GAGGAAGTGC CTAGAACCAT CATCGTGACC ACCAGAAGCC AGTACGGACT
     5521 GCCTGAGGAT GCCATCGTGT ACTGTAACTT CAACCAGCTG TACAAGATCG ACCCCAGCAC
     5581 TCTGCAGATG TGGGCCAACA TTCTGAAGCG GGTGCCCAAT TCTGTGCTGT GGCTGCTGAG
     5641 ATTTCCCGCC GTGGGCGAAC CCAATATCCA GCAGTATGCC CAGAACATGG GCCTGCCTCA
     5701 GAACAGAATC ATCTTTAGCC CCGTGGCCCC CAAAGAGGAA CACGTCCGAA GAGGACAGCT
     5761 GGCTGACGTG TGTCTGGACA CCCCTCTGTG TAATGGCCAC ACCACAGGCA TGGATGTCCT
     5821 GTGGGCTGGA ACCCCTATGG TCACAATGCC TGGCGAAACC CTGGCCTCTA GAGTGGCTGC
     5881 ATCTCAGCTG ACATGTCTGG GATGTCTCGA GCTGATCGCC AAGAACAGGC AGGAATACGA
     5941 GGACATTGCC GTGAAGCTGG GCACCGATCT GGAATACCTG AAAAAAGTGC GGGGAAAAGT
     6001 GTGGAAGCAG CGGATCTCCT CCCCCCTGTT CAACACCAAG CAGTATACCA TGGAACTGGA
     6061 AAGACTGTAC CTCCAGATGT GGGAGCACTA CGCTGCCGGC AACAAGCCCG ATCACATGAT
     6121 CAAGCCTGTG GAAGTGACCG AGAGCGCCga aaacttgtac tttcaaggct CCAGCTTTCT
     6181 TGTACAAAGT GGTATGAGCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT
     6241 GGAACTGGAT GGCGACGTGA ACGGCCACAA GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA
     6301 CGCCACAAAT GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
     6361 TTGGCCTACC CTCGTGACCA CACTGACCTA CGGCGTGCAG TGCTTCAGCA GATACCCCGA
     6421 CCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACG TGCAGGAACG
     6481 GACCATCAGC TTCAAGGACG ACGGCACCTA CAAGACCAGA GCCGAAGTGA AGTTCGAGGG
     6541 CGACACCCTC GTGAACCGGA TCGAGCTGAA GGGCATCGAT TTCAAAGAGG ACGGCAACAT
     6601 CCTGGGCCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTGTACATCA CCGCCGACAA
     6661 GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGGCAC AACGTGGAAG ATGGCAGCGT
     6721 GCAGCTGGCC GACCACTACC AGCAGAACAC CCCCATCGGA GATGGCCCCG TGCTGCTGCC
     6781 CGACAACCAC TACCTGAGCA CCCAGAGCGT GCTGAGCAAG GACCCCAACG AGAAGCGGGA
     6841 CCACATGGTG CTGCTGGAAT TCGTGACCGC CGCTGGCATC ACCCACGGCA TGAGCGGCCT
     6901 GAACGACATC TTCGAGGCCC AGAAAATCGA GTGGCACGAG CACCACCATC ATCACCATCA
     6961 CCACTGATGA TTCGAAAAAT AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA
     7021 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT
     7081 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA
     7141 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT
     7201 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT
     7261 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG
     7321 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC
     7381 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG
     7441 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT
     7501 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT
     7561 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT
     7621 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT
     7681 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC
     7741 AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG
     7801 CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA
     7861 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC
     7921 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA
     7981 AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG
     8041 TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT
     8101 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA
     8161 GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC
     8221 CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG
     8281 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC
     8341 AAAATATTAA CGCTTACAAT TTAG
//

